polyclonal goat anti human il1ri  (R&D Systems)

Journal: PLoS ONE

Article Title: Crucial Role of IL1beta and C3a in the In Vitro -Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma

doi: 10.1371/journal.pone.0116772

Figure Lengend Snippet: A) MSC on glass slides were stained with an anti-IL1RI antibody (left) or the non-specific IgG isotype control (right). Gene expression levels of B) PTGES, C) TSG6 and F) MMP1 were measured with quantitative real-time PCR and normalized to those of HPRT1 following total RNA isolation 24 h post stimulation. D) PGE 2 and E) TSG6 levels in supernatants were detected with the Biotrend PGE2 Enzyme Immunoassay Kit and TSG6 ELISA. MSC were seeded at 5.2*10 3 cells/cm 2 and stimulated with the PTC or IL1beta after the anti-IL1RI antibody or the non-specific (ns) IgG isotype control had been added. Scatter plots and mean from up to 4 donors are presented.

Article Snippet: In experiments, in which the binding of IL1beta to the IL1 receptor I (IL1RI) was inhibited to suppress IL1beta signaling, 2 μg/ml of the polyclonal goat anti-human IL1RI antibody (AF269, R&D Systems, Wiesbaden, German) was added to the medium prior to addition of IL1beta or the PTC.

Techniques: Staining, Control, Gene Expression, Real-time Polymerase Chain Reaction, Isolation, Enzyme-linked Immunosorbent Assay